Rice fields are major habitats of wildlife and they can substitute natural wetlands. Therefore, various ecological studies have been conducted for the conservation and management of rice fields worldwide. Amphibians are one of the representative taxonomic groups using rice fields as their breeding sites and their populations are rapidly decreasing due to habitat destruction and water pollution. This study examined the environmental characteristics of Wonsan salamander(Hynobius leechii)’s breeding sites in rice fields in the mid-western region of South Korea and evaluated if they were suitable breeding sites for Wonsan salamanders. The results of this study showed that Wonsan salamander used eight sites out of the surveyed 40 rice field sites in the mid-western region of South Korea for their breeding. To achieve study objectives, this study evaluated the breeding of Wonsan salamanders, landscape factors, atmospheric factors, physical factors, and aquatic factors from March to April in 2016 and 2017. The Wonsan salamander’s breeding sites had smaller rice field areas and larger forest areas, were farther from the coast, and were located at higher altitude compare to Wonsan salamander’s nonbreeding sites. These breeding sites also had low EC, TDS, and NaCl. It was found that all environmental factors affecting the breeding site selection greatly were landscape factors. In conclusion, rice fields near forests in the mid-western region of South Korea had an environment suitable for Wonsan salamander breeding and their aquatic environment also met the recommended conditions for amphibian species’ breeding. However, Wonsan salamanders didn’t breed in rice fields on plains located near the coast and the EC and TDS of them were high, which could adversely affect the inhabitation of amphibian species.